DNASIZE: This computer program has been developed for computing
the size of DNA fragments from their electrophoretic mobilities
using a graphical method. This program can also be used to compute
the size of proteins or polypeptides from SDS-PAGE. In addition to
computing the size of DNA fragments and protein size the program
also allows one to present the comparison of standard curves obtained
from gel electrophoresis (or SDS-PAGE). The program is written in
C and can be run on IBM PC or a compatible computer under MS-DOS
version 2.0 or higher, and requires a CGA, EGA or VGA card. Click to down load t
his program.
Reference:Raghava, G.P.S. (1994) Improved estimation of DNA
fragment lengths from gel electrophoresis. Biotechniques 17: 100-104.
GMAP: A comprehensive computer program GMAP, has been developed for
mapping the potential restriction endonuclease (R. E.) sites in
non-ambiguous DNA sequence, predicting the number and type of
mutations required to introduce unique R.E. sites in the non-ambiguous,
searching all R.E. sites in ambiguous DNA sequence obtained by
reverse translation of a given amino acid sequence; iv) searching R.E.
sites in DNA sequence obtained from reverse translation of amino acid
sequence employing user-defined codon usage. These facilities allow the program to be employed for the design of synthetic genes as well
as the modular redesign of gene.Click to down load this program.
Reference: Raghava, G.P.S. and Sahni, G. (1994) GMAP: a
multipurpose computer program to aid synthetic
gene design, cassette mutagenesis and
introduction of potential restriction sites into
DNA sequences. Biotechniques 16: 1116-1123.
DNAOPT: DNAOPT computer program has been developed to assist the users
in optimizing the conditions of gel electrophoresis and SDS-PAGE. Click to down load this program.
Reference:Raghava, G. P. S. DNAOPT : A computer program
to aid optimization of gel conditions of DNA gel
electrophoresis and SDS-PAGE. Biotechniques 18:
274-81.
ELISAeq: A computer program is developed which utilizes a graphical
method for determining the concentration of either the antibody or
the antigen from ELISA data. This computer program also calculate
essential parameter the signal, capacity and flatness (constants used
in hyperbolic regression), for ELISA optimization.This program is develoed in GW-BASIC at IBM-PC under DOS. Click to down load this program.
Reference:Raghava, G.P.S., Joshi, A.K. and Agrewala, J.N. (1992)
Calculation of antibody and antigen concentrations from
ELISA data using a graphical method. J. Immunol.
Methods 153, 263-264
Ab-Affi: A simple and reliable method based upon law of mass action
for calculating affinity of a monoclonal antibody using non-competitive
ELISA, has been developed. This program is develoed in GW-BASIC at IBM-PC under DOS. Click to down load this program.
Reference: Raghava, G.P.S. and Agrewala, J.N. (1994) Method for
determining the affinity of monoclonal antibody using
non-competitive ELISA : A computer program. Journal of
Immunoassay 15: 115-128.
IL4IFNG: A computer program for computing of murine interleukin-4 and
interferon-gamma by exploiting the unique abilities of these
lymphokines to induce the secretion of IgG1 AND IgG2a has been
developed.This program is develoed in GW-BASIC at IBM-PC under DOS.Click to down load this program.
Reference:Agrewala, J.N., Raghava, G.P.S., Mishra, G.C. (1993)
Measurement and computation of murine interleukine-4
and interfron-gamma by exploiting the unique abilities of
these lymphokines to induce the secretion of IgG1 and
IgG2a. Journal of Immunoassay 14, 83-97
